Bone marrow-derived MPs (BMDMs) were isolated from Aif1 -- mice for study in culture.
In vitro, lack of AIF1 reduced BMDM survival, phagocytosis, and efferocytosis.
Mechanistically, AIF1 supported activation of the NF-B pathway and expression of related target genes involved in stress response, inflammation, and apoptosis.
In inflammatory settings such as atherosclerosis, these AIF1-dependent activities serve to clear cellular and other debris and limit necrotic core expansion, and may oppose lesion destabilization.
Right panel, representative brachiocephalic artery (BCA) lesions from ApoE (n8) and Aif1; ApoE (n10) mice after 18 weeks of high fat feeding stained with ORO.
Teradata client for mac 15B) Left panel; quantification of the en face Sudan IV staining of aortae.
Right; quantification of the ORO staining of BCAs.
C) Left panel, representative sections of hematoxylin and eosin-stained aortic root sections from ApoE (n10) and Aif1; ApoE (n10) mice.
The boxed areas are expanded in the middle panels (necrotic areas; bar, 200 M).
D) Quantification of aortic root lesion size from ApoE or Aif1; ApoE mice.
E) Quantification of necrotic (acellular) area from ApoE or Aif1; ApoE mice.
F) Quantification of collagen content from ApoE or Aif1; ApoE mice.
Survival was assessed and quantified with a LIVEDEAD viability kit.
C) Wt and Aif1 BMDMs were synchronized and incubated with the indicated numbers of Alexa 488-zymosan particles per cell (bar, 25 M).
D) The percentage of internalized zymosan particles per cell was measured by FACS.
E) FACS quantification of the number of F480 cells recruited to the site of inflammation in wt (n5) or Aif1 (n6) mice.
F) FACS quantification of F480 macrophages isolated from wt (n5) or Aif1 (n6) that have ingested TAMRA-labeled apoptotic Jurkat T-cells.
B) Quantification of phosphorylated p65 Ser536 protein levels, relative to total p65 levels.
C) Quantification of phosphorylated p65 Ser276 protein levels, relative to total p65 levels.
D) RAW 264.7 cells were transfected with 2 gml of pcDNA3.1- Aif1 or control pcDNA3.1.
Ser536 and Ser276.
E) Quantification of phosphorylated p65 Ser536 and Ser276 protein levels, relative to total p65 levels.